Immunogene expression analysis in betanodavirus infected-Senegalese sole using an OpenArray® platform

The transcriptomic response of Senegalese sole (Solea senegalensis) triggered by two betanodaviruses with different virulence to that fish species has been assessed using an OpenArray® platform based on TaqMan™ quantitative PCR. transcription 112 genes per sample evaluated at sampling times in organs (head kidney and eye/brain-pooled samples). Those were involved several roles or pathways, such as viral recognition, regulation type I (IFN-1)-dependent immune responses, JAK-STAT cascade, interferon stimulated genes, protein ubiquitination, virus responsive complement system, inflammatory response, other system effectors, T-cell proliferation, proteolysis apoptosis. highly virulent isolate, wSs160.3, a wild reassortant containing RGNNV-type RNA1 SJNNV-type RNA2 segments, induced the expression higher number both tested than moderately strain, recombinant harbouring mutations protruding domain capsid protein. differentially expressed was 2 days after infection isolate 3 post-inoculation. also elicited exacerbated 1 which, instead protecting against infection, increases disease severity induction apoptosis inflammation-derived immunopathology, although inflammation seems be modulated system. Furthermore, results derived from this study suggest potential important role for some high virus, rtp3, sacs isg15. On hand, mutant does not induce probably due altered recognition host, which is supported pathway, involving myd88 tbkbp1.